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Registros recuperados: 27
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Action of pesticides to Metarhizium anisopliae in soil Neotropical Entomology
Mochi,Dinalva A.; Monteiro,Antonio C.; Barbosa,José C..
The present study aimed to analyze the action of some acaricides, fungicides, insecticides and herbicides containing different active ingredients on Metarhizium anisopliae Metsch. (Sorokin) inoculated into autoclaved soil. The action of the pesticides was evaluated based on the fungal respiratory activity. The first assessment was done at 48h after inoculation. The pesticides were then added and respiratory activity was determined nine times every 48h and an additional five times every 4 days. Except for the fungicides, no significant effect (P > 0.05) of the pesticides on M. anisopliae was observed. A reduction in CO2 production was observed for the mancozeb treatment from day 4 to day 6 of incubation, and for tebuconazol between days 4 and 6, 8 and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Entomopathogenic fungus; Integrated management; Respiratory activity.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000600013
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Biocontrol of pigeon tick Argas reflexus (Acari: Argasidae) by entomopathogenic fungus Metarhizium Anisopliae (Ascomycota: Hypocreales) BJM
Tavassoli,Mosa; Pourseyed,Seyed Hassan; Ownagh,Abdulghaffar; Bernousi,Iraj; Mardani,Karim.
The pigeon tick Argas reflexus is a pathogen-transmitting soft tick that typically feeds on pigeons, but can also attack humans causing local and systemic reactions. Chemical control is made difficult due to environmental contamination and resistance development. As a result, there is much interest in increasing the role of other strategies like biological control. In this study, the efficacy of three strains (V245, 685 and 715C) of entomopathogenic fungus Metarhizium anisopliae for biological control of three life stages of pigeon tick A. reflexus including eggs, larvae, engorged and unfed adults was investigated under laboratory conditions. Five concentrations of different strains of M. anisopliae ranging from 10³ to 10(7) conidia/ml were used. All...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Argas reflexus; Pigeon tick; Biological control; Entomopathogenic fungus; Metarhizium anisopliae.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400030
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Cage and field assessments of Beauveria bassiana-based Mycoinsecticides for Myzus persicae Sulzer (Hemiptera: Aphididae) control in cabbage Neotropical Entomology
Michereff Filho,M; Oliveira,SOD; de Liz,RS; Faria,M.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomopathogenic fungus; Formulation; Microbial control.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000400010
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Compatibility of Beauveria bassiana with acaricides Neotropical Entomology
Oliveira,Renato C. de; Neves,Pedro M.O.J..
The in vitro compatibility of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuil. with 12 acaricide formulations was evaluated. The active ingredients Clorfenapyr, Fenpyroximate, Amitraz, Acrinathrin, Hexythiazox, Abamectin, Pyridine, Dimethoate, Pyridaphethion, Fenbutatin Oxide, Azocyclotin and Cyhexatin were tested in three different concentrations (mean concentration - MC, half MC and twice the MC). The formulations tested affected conidial germination, vegetative growth and sporulation of the fungus in different levels. The formulations more compatible with B. bassiana were Avermectin and the pyrethroids. The acaricides belonging to the organophosphate and organostanic chemical groups, however, drastically affected conidial germination as well...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Selectivity; Fungitoxicity; Entomopathogenic fungus; IPM.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000300013
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Compatibility of the fungus Beauveria Bassiana (Bals.) Vuill. (Deuteromycetes) with extracts of neem seeds and leaves and the emulsible oil Neotropical Entomology
Depieri,Rogério A.; Martinez,Sueli S.; Menezes Jr.,Ayres O..
The compatibility of a commercial formula of emulsible neem oil (Azadirachta indica A. Juss.) and of aqueous extracts of neem seeds and leaves with Beauveria bassiana (Bals.) Vuill. was evaluated in vitro. Three experiments were conducted to evaluate the effect of each product on the fungus vegetative growth and on conidia production and viability. The products were incorporated to a culture medium (BDA+E) and distributed into petri dishes, in the following concentrations: 0.15%; 1.5% and 15% (leaf aqueous extract), 1%; 2% and 4% (seed aqueous extract) and 0.5%; 1% and 1.5% (emulsible oil). Vegetative growth and conidia production were the basis for characterization of the aqueous extracts of seeds and leaves and of the emulsible oil, using the T...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azadirachta indica; Selectivity; Biological control; Entomopathogenic fungus.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000400010
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Differential pathogenicity of Metarhizium anisopliae and the control of the sugarcane root spittlebug Mahanarva fimbriolata BABT
Tiago,Patricia Vieira; Souza,Hilton Marcelo de Lima; Moysés,Juliano Borsato; Oliveira,Neiva Tinti de; Lima,Elza Áurea de Luna Alves.
In order to assess the effectiveness of Metarhizium anisopliae var. anisopliae (Metschnikoff) Sorokin isolates in controlling the sugarcane root spittlebug Mahanarva fimbriolata (Stal) (Hemiptera: Cercopidae), nine isolates obtained from a single geographical region were studied. 'Confirmed cumulative' and 'corrected cumulative' spittlebug mortality rates were measured for each of the isolates. Based on the confirmed mortality curve, the isolates URM5946, URM5951 and URM6033 were considered to be potentially the most effective in a biological control program for M. fimbriolata.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Entomopathogenic fungus; Metarhizium anisopliae; Mahanarva fimbriolata.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300002
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Differentiation of the entomopathogenic fungus Metarhizium flavoviride (Hyphomycetes) Rev. Microbiol.
Xavier-Santos,Solange; Magalhães,Bonifácio; Lima,Elza A. Luna-Alves.
The differentiation of a Brazilian isolate of Metarhizium flavoviride (CG 423), a promising candidate for the biocontrol of grasshoppers, was investigated. Conidia were spread onto solid medium (1% yeast extract, 2.8% agar, 96.2% distilled water), incubated at 28°C and observed during 26 h. Germination initiated as conidia size increased from 5.3 (±0.6) x 3.1 (±0.3) µm (0 h incubation) to 8.1 (±0.2) x 6.1 (±0.2) µm (8 h incubation). Germ tubes started to appear after 10 h incubation showing a high degree of multipolarity. Twenty six hours after inoculation, hyphal differentiation and anastomosis among hyphae from adjacent conidia were recorded. Appressoria were formed only from conidia incubated in liquid medium containing minimum concentration of yeast...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Hyphomycetes; Entomopathogenic fungus; Germination; Appressorium.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100009
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Effect of Disinfectants and Pesticides Used in Poultry Houses on Beauveria Bassiana (Bals.) Vuill.fungus Rev. Bras. Ciênc. Avic.
Alves,LFA; Mamprim,AP; Formentini,MA; Martins,CC; Pinto,FG.
ABSTRACT This study aimed at evaluating the effect of the use of disinfectants and insecticides recommended for the control of pathogens and insects in poultry houses on the biological parameters of the entomopathogenic fungus Beauveria bassiana strain Unioeste 4. Disinfectants and insecticides were used at recommended concentration (RC), half the recommended concentration (0.5 RC), and twice there commended concentration (2RC). All treatments were sprayed on the fungus previously inoculated on PDA culture media. Germination, colony forming unit (CFU), vegetative growth and conidial yield were evaluated. Germination was the most affected parameter by insecticides, with reduction of up to 25% using Couro Limpo and Cypermil products. Cypermil also reduced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomopathogenic fungus; Compatibility; Associated control; Animal production.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200283
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Food consumption of Rhammatocerus schistocercoides Rehn (Orthoptera: Acrididae) infected by the fungus Metarhizium flavoviride Gams & Rozsypal Anais da SEB
Faria,Marcos R. de; Almeida,Danylo de O.; Magalhães,Bonifácio P..
Foliage consumption by nymphs and adults of the grasshopper Rhammatocerus schistocercoides Rehn infected with the fungus Metarhizium flavoviride Gams & Rozsypal was evaluated. Sixth and eighth instar nymphs and female adults consumed on average (±SEM) 6.9 ± 0.36 cm² (60.7 ± 2.92 mg of dry matter), 11.0 ± 1.35 cm² (74.9 ± 9.24 mg d.m.), and 16.0 ± 0.50 cm² (126.5 ± 6.35 mg d.m.) of sugarcane leaves per day, equivalent to 85.4, 35.9 and 41.9% of the insect body weights, respectively. Eighth instar nymphs and female adults inoculated with M. flavoviride (5,000 conidia / insect) ingested less food from day three onward and showed at day 10 an average consumption 74.5 and 45.6% lower than control insects, respectively. Adult females topically treated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Feeding reduction; Foliage consumption; Entomopathogenic fungus.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591999000100010
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Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partial beta-tubulin gene sequences Genet. Mol. Biol.
Tigano,Myrian S.; Adams,Byron; Maimala,Saowanit; Boucias,Drion.
Amplified fragment length polymorphism (AFLP) was used to investigate polymorphism among 43 Hirsutella thompsonii isolates (33 from Thailand) obtained from various mite species. The outgroups were an unidentified Hirsutella isolate along with Hirsutella nodulosa and Hirsutella kirchneri. Phylogenetic analyses of the AFLP data showed significant variation among isolates and the existence of three H. thompsonii clades. We also investigated the isolates using PCR with specific primers for the Hirsutella exotoxin gene Hirsutelin A (HtA) and 18 of these isolates were used for sequencing of the partial beta-tubulin gene. Phylogenetic analyses of beta-tubulin sequences showed two distinct H. thompsonii clades, one of which included AFLP clades I and II. For both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomopathogenic fungus; Mites; Molecular markers.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400022
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Horizontal transmission and effect of the temperature in pathogenicity of Beauveria bassiana against Diatraea saccharalis (Lepidoptera: Crambidae) BABT
Svedese,Virgínia Michelle; Lima,Elza Áurea de Luna Alves; Porto,Ana Lúcia Figueiredo.
The aim of this work was to evaluate the effect of temperature on the pathogenicity of Beauveria bassiana against Diatraea saccharalis and evaluate the horizontal transmission capacity among the sugarcane borer larvae. The strains were most pathogenic at 26ºC, followed by 32ºC than at 20ºC, with respective mortalities of 100, 50 and 30.3%. The lethal time was also affected by the temperature, with the shortest LT 50 observed at 26ºC (2.2 days) and the longest at 20ºC (16.2 days). The URM2915 strain appeared most effective in all the experiments as having potential for deployment in biological control programs for the sugarcane borer. Results in horizontal transmission of B. bassiana showed that this procedure could also be adopted as control strategy for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sugarcane borer; Lepidoptera; Entomopathogenic fungus; Biological control; Autodissemination.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300009
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Mechanism of infection and colonization of Rhipicephalus sanguineus eggs by Mertarhizium anisopliae as revealed by scanning eletron microscopy and histopathology BJM
Garcia,Marcos Valerio; Monteiro,Antonio Carlos; Szabo,Matias Juan Pablo; Prette,Nancy; Bechara,Gervásio Henrique.
The present work aimed to verify the penetration pattern of the Metarhizium anisopliae fungus into Rhipicephalus sanguineus tick eggs, as well as the lesions occurred inside the egg. The fungus adherence and penetration were studied by scanning electron microscopy and the action of fungus in the internal tissues was evaluated through conventional histological sessions. For the observation of these events, experimental infections were performed in 11 groups of R. sanguineus eggs containing 25 mg each. The eggs were bathed during 3 minutes under manual shaking in a 10(8) conidia/mL suspension. The bath was performed only in the suspension vehicle for the control groups. The eggs were processed for histopathological analysis and scanning electron microscopy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Microbial control; Entomopathogenic fungus; Ticks.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400012
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Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates Scientia Agricola
Azevedo,Andréia Cristiane Souza; Furlaneto,Márcia Cristina; Sosa-Gómez,Daniel Ricardo; Fungaro,Maria Helena Pelegrinelli.
ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paecilomyces fumosoroseus; Bemisia; RAPD; Biological control; Entomopathogenic fungus.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400021
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Pathogenicity of Metarhizium anisopliae for Ceratitis capitata (Wied.) (Diptera: Tephritidae) in soil with different pesticides Neotropical Entomology
Mochi,Dinalva A.; Monteiro,Antonio C.; De Bortoli,Sergio A.; Dória,Háyda O.S.; Barbosa,José C..
This research intended to investigate if the presence of pesticides in the soil could affect the pathogenicity of Metarhizium anisopliae Metsch. (Sorokin) for Ceratitis capitata (Wied.) and assess the effect of conidia application as suspension or dry conidia. The fungicides chlorothalonyl and tebuconazol, the acaricide abamectin, the insecticide trichlorfon, and the herbicide ametrin were applied at the manufacturer-recommended doses. Soil samples were placed in glass flasks and were given the fungus as conidial suspension or dry. After pesticide application, 20 3rd-instar larvae were placed in the soil. The flasks were sealed with voile fabric and incubated at 27 ± 0.5ºC for nine days, until adult emergence; incubation continued for four more days at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Microbial control; Entomopathogenic fungus; Fruit fly.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000300014
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Patogenicidad de Metarhizium anisopliae (Metch.) Sorokin y Beauveria bassiana (Bals.) Vuill sobre Periplaneta americana (L.) y su sinergismo con el ácido bórico Colegio de Postgraduados
Hernández Ramírez, Gabriela.
Los hongos entomopatógenos Beauveria bassiana (Balsamo) Vuillemin 1912 y Metarhizium anisopliae (Metschnikoff) Sorokin 1976 fueron aplicados sobre ninfas y adultos de la cucaracha Periplaneta americana (Linnaeus, 1758) en condiciones de laboratorio (1.1x106 conidia, temperatura 24 ±2°C y humedad relativa, HR, 50±10%). Los aislamientos probados mostraron bajo potencial patogénico y porcentajes de mortalidad significativamente diferentes entre aislamientos. Además se observó menor mortalidad en adultos que en ninfas, en las que se presentaron cambios de conducta a la aplicación de la suspensión conidial. Los dos aislamientos más virulentos fueron seleccionados para ser probados en tres grupos de edades representativos de las etapas de desarrollo de P....
Tipo: Tesis Palavras-chave: Cucaracha americana; Cebos; Hongos entomopatógenos; Control integrado; Maestría; Entomología y Acarología; American cockroach; Baits; Entomopathogenic fungus; Integrated control.
Ano: 2007 URL: http://hdl.handle.net/10521/1309
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Polymorphism of the grasshopper Schistocerca pallens (Thunberg) (Orthoptera: Acrididae) and its natural pathogen Metarhizium flavoviride Gams & Rozsypal (Hyphomycetes), revealed by RAPD analysis Anais da SEB
Silveira,Eni B.; Al-Janabi,Salah M.; Magalhães,Bonifácio P.; Carvalho,Luiz J.C.B.; Tigano,Myrian S..
The genetic variability of 27 individuals from two distinct populations (Northeast and Central Brazil) of the grasshopper Schistocerca pallens (Thunberg) and eight isolates of its natural pathogen, the fungus Metarhizium flavoviride Gams & Rozsypal, was investigated using RAPD analysis. For the grasshoppers, ten different 10-mer oligonucleotide primers of arbitrary sequence were selected for analysis, resulting in 79 scorable binary characters. The program RAPDDIP applied to S. pallens revealed nucleotide diversity of 2.3 and 2.2% for the populations from Rio Grande do Norte (Northeast Brazil) and Federal District (Central Brazil), respectively. These values indicate the presence of high genetic variability within these populations. Conversely, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Genetic variability; Entomopathogenic fungus; Biological control; Molecular markers.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0301-80591998000100012
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Population density of Beauveria bassiana in soil under the action of fungicides and native microbial populations Agronomy
Soares, Flávia Barbosa; Monteiro, Antonio Carlos; Babosa, José Carlos; Mochi, Dinalva Alves.
This study investigated whether populations of naturally-occurring soil bacteria, fungi and actinomycetes influence the effect of fungicides on the survival and growth of Beauveria bassiana. The toxicity of methyl thiophanate, pyraclostrobin, mancozeb and copper oxychloride at the recommended doses was analyzed in culture medium and in soil inoculated with fungus at various time points after addition of fungicides. All fungicides completely inhibited the growth and sporulation of B. bassiana in the culture medium. The fungicides were less toxic in soil, emphasizing the action of the microbial populations, which interfered with the toxic effects of these products to the fungus. Actinomycetes had the greatest influence on the entomopathogen, inhibiting it or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências Agrárias; Microbiologia; Biological control; Entomopathogenic fungus; Soil microorganisms; Compatibility; Toxicity. Ecologia de Micro-organismos; Fungo entomopatogênico; Polulações nativas do solo; Micro-organismos do solo.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32816
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Populational parameters of Spalangia endius Walker (Hymenoptera: Pteromalidae) on Pupae of Musca domestica L. (Diptera: Muscidae) treated with two strains of Beauveria bassiana (Bals.) Vuil. (Deuteromycetes) Neotropical Entomology
Lecuona,Roberto; Crespo,Diana; La Rossa,Francisco.
The parasitoid Spalangia endius Walker is an efficient controller of Dipteran pupae, such as Musca domestica L. The entomopathogenic fungus Beauveria bassiana (Bals.) Vuil. is a regulator of insect populations, including these synanthropic pests. The aim of this work was to explore the possibilities of utilizing both agents in a combined form for the biocontrol of the domestic fly. Recently formed M. domestica pupae were inoculated by immersion in conidia suspension (10(8) conidia/ml) with two strains of B. bassiana (Bb6 and Bb10). The inoculated pupae were offered to the female parasitoid. In one bioassay they were offered pupae inoculated a single day and in other, pupae inoculated the following day as well. In both bioassays non inoculated (control)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entomopathogenic fungus; Biological control.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000400010
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Protease production during growth and autolysis of submerged Metarhizium anisopliae cultures Rev. Microbiol.
Braga,Gilberto U.L.; Destéfano,Ricardo H.R.; Messias,Claudio L..
The growth and autolysis of two strains of the entomopathogenic deuteromycete fungus Metarhizium anisopliae var. anisopliae were evaluated in medium containing casein or glucose as carbon source. Parameters such as economic coefficient and degree of autolysis were determined for each strain. Protease production was determined throughout the growth and autolysis phases of the cultures on medium under conditions of protease induction (in the presence of casein as sole source of carbon and nitrogen). The fungus was shown to utilize casein as a carbon/energy source in a more efficient manner than glucose. The autolysis shown by the strains was intense under both types of growth conditions, reaching up to 62.7% of the dry mass produced and started soon after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metarhizium anisopliae; Protease production; Fungal growth; Fungal autolysis; Entomopathogenic fungus.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200004
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Pulsed field gel electrophoresis reveals chromosome length and number differences in Brazilian strains of Metarhizium Anisopliae BABT
Kava-Cordeiro,Vanessa; Queiroz,Marisa Vieira de; Pizzirani-Kleiner,Aline Aparecida; Azevedo,João Lúcio.
Electrophoretic karyotypes of eight wild-type strains of Metarhizium anisopliae var. anisopliae were obtained by pulsed-field gel electrophoresis. These strains were isolated from insects of six different Brazilian states. The chromosomal DNA molecules of three strains were separated into seven bands and of five strains into eight bands. Chromosome length polymorphisms were also observed. The size of the chromosomal DNA of all strains varied between 7.7 and 0.9 Mb using the Aspergillus nidulans chromosomes as size standards. The total genome size of these strains was estimated in at least 29.7 Mb. Some correlations between differences in karyotype and occurrence of parasexual cycle likewise the host specificity were discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metarhizium anisopliae; Pulsed-field gel electrophoresis; Chromosome polymorphisms; Parasexual cycle; Entomopathogenic fungus.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100001
Registros recuperados: 27
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